Research Article Open Access

A non-tissue culture-dependent approach for genetic transformation of Marchantia polymorpha L.

Abstract

Marchantia polymorpha L. is a prime example of an early terrestrial plant. It acts as a desirable model species for biological research as a result of its short growth cycle, predominant haploid gametophyte, simultaneous sexual and asexual propagation, etc. In this study, a non-tissue culture-dependent approach to the genetic transformation of M. polymorpha L. was developed. This approach comprises immersion of explants (thalli or mature gemmae obtained in the field conveniently, with no pre-culture) in the solution of Agrobacterium, inoculation on soil simply, and screening by spraying selection solution directly on them. As a result, over-expression of MpCCAAT-NFYB5 (Mapoly0053s0055.1) and corresponding phenotypic changes were observed. Furthermore, it was demonstrated that non-chimera transformants could be obtained rapidly through four cycles of gemmae reproduction. This method would facilitate further molecular biological research on this specific plant

Lan Zhang, Ning Xu, Jianming Man, Luo Rui*

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