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Adventitious shoot regeneration in Sesame (Sesamum indicum L.) (Pedaliaceae) via deembryonated cotyledonary explants

Abstract

Adventitious shoot regeneration in sesame, via de-embryonated cotyledonary explants, was attempted using six diverse genotypes; two were land races (Kankapura Local – KNL and Tamil Nadu Local- TNL), three were released varieties (DS-1, DSS-9 and WII) and a breeding line (RT-273). Three media compositions (½ MS basal media supplemented with 20 μM TDZ + 2.5 μM IAA and with or without 25 μM BAP and full MS basal media supplemented with only 25 μM BAP) were used of which highest shoot regeneration (100%) with 8.15 and 7.37 shoot numbers per cotyledon was achieved in RT-273 in both the combinations (½ MS with 20 μM TDZ+ 2.5 μM IAA+ 25 μM BAP or without BAP) followed by DS-1(100% and 95.55% with 6.15 and 3.89), KNL (88.88% and 7.55) and TNL (88.88% and 5.73) respectively in ½ MS with 20 μM TDZ+ 2.5 μM IAA+ 25 μM BAP. MS with 25 μM BAP alone failed to induce shoot regeneration.

Shilpa V. Malaghan, Ramappa Lokesha, Revadi Savitha and Ajjanavara R. G. Ranganatha

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