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Antagonistic Activity by Microbial Blended CuO NPs of Endophytic Actinomycetes Isolated from Triticum Vulgare against Plant Pathogens

Abstract

For the creation of nanoparticles, two endophytic actinomycetes were used, both of which belonged to the Streptomyces species. The 16SrRNA gene sequence was used to identify these species. Streptomyces noursei (A-1) and Streptomyces fradiae were identified as the two species (A-2). Cu oxide nanoparticles were made from biomass extracts of these species using the Cu SO4: 5H2O method. UV visible spectroscopy, FTIR spectroscopy, X-ray Diffraction, and transmission electron microscopy were used to characterise the nanoparticles (TEM) Surface plamon resonance (SPR) absorption band in the region of 410 to 450 nm was measured in the biosynthesis of Cu oxide nanoparticles. The Cu oxide nanoparticles produced via biosynthesis were spherical in shape and crystalline in structure. The size of Cu oxide nanoparticles produced via biosynthesis ranges from 50 to 100 nm. The crystalline nature of both A-1 and A-2 biosynthesized actinomycetal CuO nanaoparticles was revealed by XRD examination. The presence of a number of distinct bonds, including large C-O and N-H bonds, was determined using FTIR spectra.

Vinay V Chaugule*

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