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Cytomophorlogical Analysis of Urothelial Cells Among Cigarette Smokers

Abstract

This is a descriptive study carried out in Owo Town, Ondo State, Nigeria. The main purpose of carrying out this research work is to evaluate the cytomophorlogical features of urothelial cells (using papanicoulaou stain) among cigarette smokers in Owo town, Ondo State, Nigeria. Two hundred and fifty (250) subjects were used for this research work, 200 subjects were cigarette smokers while 50 subjects were non- cigarette smokers. The numbers of years of cigarette smoking were different and the numbers of cigarette sticks smoked per day were also variable among the test group. Individuals with urinary tract infection were not included in this research work and individuals with less than five (5) years of cigarette smoking were also not included in this research work. From each urine sample collected, smears were obtained from the sediments after centrifuging and were immediately fixed with a cytology-spray fixative for at least 30 minutes, before staining smears with Papanicolaou stain. The stained smears were examined under a light microscope and revealed a high cellular turnover among 70% of the test group when compared with the control group which is non-smokers, showing few normal urothelia cells. Enlargement in nuclear cytoplasm ratio, irregular nuclear borders, necrosis, cluster of cells showing dysplastic changes, moderate haemorrhage, heavy infiltrates of inflammatory cells, hyperchromatism, pleomorphysms and neoplastic transformation were among the features observed in smears of the test group. On the basis of this research work, cigarette smoking has been seen to be one of the leading causes of renal diseases. Active cigarette smoking is a major risk factor for bladder cancer. Secondhand exposure to cigarette smoke may also contribute to bladder carcinogenesis.

Ajileye, Ayodeji Blessing, 

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