Research Article Open Access

Estimation of the Erythrocytes Diameter for the Detection of the Specific Binding with Blood Group Antibodies

Abstract

Different methods of the detection of blood group antigens exist in hematology. Nevertheless hemolytic reactions still occur despite an accurate performance of standard methods of blood group detection. Binding of the antigen with antibody is fixed by the phenomenon of agglutination of erythrocytes. Since we measured the erythrocyte diameter size we calculated Relative blood cell Distribution Size (RDS) by dividing the Standard Deviation (SD) of erythrocyte size from the Mean Corpuscular Size (MCZ). The result of this straightforward equation is then multiplied by 100 to express results in percentage (%).The increase of the mean value of the erythrocytes diameter on 16.6 % with appearence of agglutinated microcytes and macrocytes testify to the specific binding of absorbing and agglutinating determinants of the erythrocytes with IgG antibodies.

Kravchun PG, Leontieva FS, Danyshchuk ZM, Ashukina NO, Dielievska VYu

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