Conference Proceeding Open Access

Evaluation of Antimicrobial Activity of N-Hexane Extract of Different Parts of Pumpkin (Seed, Leaves and Pulp)

Abstract

 The present study deals with the antimicrobial activity of different extracts of pumpkin (Cucurbita pepo L.) (Seed, Leaves and pulp) against seven microorganisms: two Grampositive bacteria, i.e. Staphylococcus aureus, Bacillus cereus; three Gram-negative bacteria, i.e. Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis and two yeasts, i.e. Candida albicans& C. parapsilosis.Results revealed that all extracts (Seed, Leaves and pulp) were very effective against P. aeruginosa while some extracts showed no inhibition against S. aureus at all tested concentration (20, 40 & 80 µg/ml).The maximum inhibition zone at 80 µg/ml concentration of nhexane pulp extract was 39 ± 0.1 mm against P. aeruginosa. The minimum concentration (MIC) for n-hexane pulp extract was 1.65 µg/ml for P. aeruginosa, leading to a conclusion that the n-hexane pulp extract of pumpkinwas found to be the most potent. Thus it can be concluded that n- hexane pulp extract of Cucurbita pepo L. was more potent as compared n- hexane seed and leaves extract of Cucurbita pepo L. 

 Harsh R. Chunara

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