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Evidence for Complex Formation of Aceclofenac with Go-Ghrita by FT-IR., In-vitro Release, DSC, X-RD, NMR and SEM.

Abstract

Present investigation had undergone the evidence for complex formation of Aceclofenac (Ace) with Go-Ghrita (GG). Aceclofenac binary mixture in (1:0.5), (1:1), (1:2), (1:3) w/w proportions were prepared by adding to molten Go-Ghrita kept over a water bath at 65-70oC with continuous stirring. Organoleptic and Physico-chemical properties of GG (procured from Magan Sangrahalaya, Wardha) were as per the specifications given in Ayurvedic Pharmacopoeia (AP) and Indian Pharmacopoeia (IP). All proportions were subjected for FT-IR and in-vitro release behaviour (SGF, PH 1.2 for 2 Hrs and SIF, PH 6.8 for 7 Hrs) and on basis of it, optimized (1:1) w/w proportions were further analyzed and characterized for Differential Scanning Calorimetry, X-ray diffraction, Scanning Electron Microscopy. Carbonyl stretching of oxyacetic acid group to slight lower frequency 1.41 % cm-1 (decrease) as well as (–C=O) linked (-OH) band stretching to slight lower frequency 0.67 cm-1 (decrease) shown in FT-IR spectra, In-vitro release of Aceclofenac 99.92± 0.11 followed by zero order release kinetics with 0.9933 R2 value from (1:1) w/w in sustenance form than remainings. Exclusion of polymorphic modification in X-RD, Slight lowering of 30C Tm and enthalpy change of 4.05 % (loss) in DSC, deviation in chemical shift (ranging from -0.044 to +0.186) as well as selective broadening of signals in NMR and entrapment of discrete and irregular shape of Aceclofenac crystals in Ace-GG binary mixture shown in SEM photographs, reflecting possible inclusion type complexation between Aceclofenac and GG.

Bawankar RD, Sakarkar DM, and Dorle AK

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