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Experimental studies and Development of Modelling equation of Quercetin from Raphanus Sativus using Soxhlet Extractor

Abstract

The radish (Raphanus sativus) species belongs to Brassicaceae family. Different parts of radish including roots, seeds and leaves are used for medicinal purposes. The objective of this work was extraction of phenolic compounds from radish and to develop a modelling equation to describe quantitatively the extraction phenomena. The present study was intended to optimization of extraction of Quercetin and its various physico-chemical parameters have been studied. For the extraction of Quercetin the optimum results were observed for the effect of different solvents, soaking time, extraction t ime with hexane, particle size, different solvent percentages, different volumes of hexane with methanol as solvent and pH where the results showed up are respectively methanol, 1day, 1 hr, 200 particle mesh size, 80% (v/v), 1:1 ratio and 6.0. The highest Quercetin concentration for optimized condition was 45μg/ml. Soxhlet extraction was carried out using methanol for different extraction time periods to verify the mathematical model proposed in this work. The final form of proposed model was Es= 1.525(1-e-0.9033t) for Quercetin, where Es = yield extract (mg of quercetin/gm of dried sample) and t= extraction time (min). The model showed good agreement with the experimental data by generating average absolute relative deviation ( AARD) of about 1.217+- 13.23% mg of quercetin per 1 gm of dried sample

Meena Vangalapati, Gnana Madhuri Y, Vidya Sagar T, Ravi Raju V and Satyadev L K

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