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Flavonoids Isolated from Onosma chitralicum of Antimicrobial Potential

Abstract

The Onosma chitralicum belonging to family Boraginaceous, has been acting as a source of traditional medicine and have cooled, laxative and anthelmintic effects. The objective of the current research work was to screen the biological potential of Onosma chitralicum and also to characterize the isolated chemical constituents of the plant. The crude extract of the plant and soluble fractions of different solvent like (n- C6H14 hexane), (CHCl3 chloroform), (EtoAc ethyl acetate), (n-BuOH butanol) and aqueous had been tested against various microbes (bacteria, fungi). Among them, the activity of (EtoAc ethyl acetate) fraction was determined by technique named column chromatography which was more potent as well as afforded compounds of 1 to 7. The results of these compounds activities showing that the activity of methanol, (EtoAc ethyl acetate) and (n-C6H14 hexane) fraction of the plant was more than the fraction of (n- BuOH butanol), (CHCl3 chloroform) and aqueous fractions against bacterial strains. The methanolic/crude extract activity against A. flavus (a fungal stain) were recorded as 65 mm that was excellent. The zone of inhibition of the compounds 1, 4, 5 and 6 were 7 mm, 9 mm, 3 mm, and 6 mm, respectively, so these compounds were more potent antibacterial than compound 2, 3 and 7. Against the tested fungal strain all the isolated compounds 1 to 7 were active. Compounds 2, 3 and 7 were less active than compounds 1, 4, 5 and 6 against the tested fungal strains. Fraction of (EtoAc ethyl acetate) was most active against the selected microbes and from (EtoAc ethyl acetate) fraction compounds 1 to 7 were isolated as well as act as an antimicrobial agent.

Shakeel Ahmad Khan, Ijaz Ahmad Fozia, Syed Anis Ali Shah, Shakeel Ahmad, Najeeb Ullah, Ibrahim Khan

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