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GC-MS analysis of Indigofera tinctoria and its protective effect on noise induced behavioral and biochemical alterations in rats

Abstract

The aim of the present study is to investigate GC-MS analysis and protective effect of Indigofera tinctoria (I.tinctoria) on noise induced behavioral and biochemical changes in wistar albino rats. Noise stress was done by broadband white noise generator, 100 dBA, 4h daily for 15 days and Indigofera tinctoria (300 mg/Kg .b.w.) administered orally. Seven compounds were isolated via GC-MS analysis, out of which l-(+)-Ascorbic acid 2,6-dihexadecanoate, N-(3-(3,4,5-trimethoxyphenyl) propionyl) piperidin-2-one and 3,6-diacetyl-9-ethylcarbazole have reported to exhibit antioxidant capacity. Upon exposure to the noise-stress, a significant (P? 0.001) increase in immobilization with decrease in rearing, grooming, and ambulation behavior are seen in open field. This outcome suggest that sub-acute stress affect locomotor activity in rat. Sub-acute noise stress significantly increased the level of glutathione peroxidase, lipid peroxidation, nitric oxide, glutathione S transferase and decreased the level of superoxide dismutase, catalase, reduced glutathione, glutathione reductase, Vitamin C, protein thiols, suggesting that oxidative imbalance in immune organs when expose to sub-acute noise. However, Oral administration of I.tinctoria significantly prevented noise induced behavioral and biochemical changes. These results concluded that I.tinctoria supplementation could scavenge the noise induced free radical generation, suggesting its antioxidant property and can be regarded as an antistressor.

Sakthivel Srinivasan, Wankupar Wankhar, Sheeladevi Rathinasamy, Ravindran Rajan*

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