Research Article Open Access

Genetic Diversity of Flacourtia rukam Zoll. & Moritzi a Local Fruit Tree Using Random Amplified Polymorphic DNA Markers

Abstract

Flacourtia rukam, Zoll and Moritzi (Salicaceae) is a local fruit tree that is rarely found in various provinces in Indonesia including West Sumatra which is known to be rich in biodiversity. Therefore, an assessment of genetic diversity has been carried out at three locations under different conditions in West Sumatra using the RAPD technique with 18 primers. The results showed that five primers (OPA-01, OPA-03, OPA-13, OPA-16, OPB-10) exhibited DNA polymorphisms. The value of genetic diversity in the Matur population was the highest (H=0.1883, I=0.2770), while the Lubuk Alung population had the lowest genetic diversity value (H=0.0230, I=0.0341). Genetic diversity between populations was higher (Dst=0.1704) compared to the value of genetic differentiation of 0.5710. The PCoA analysis shows that the Matur genetic population was widely distributed, while in Aripan it was moderate, and in Lubuk Alung it was narrowest. This study concludes that the population of F.rukam in the Matur area can be used as a gene pool and source of germplasm.

Jihan Fadillah*, Mansyurdin, Tesri Maideliza

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