Research Article Open Access

In Vitro Flowering and Rapid Propagation of Physalis Minima Linn. - A Medicinal Plant

Abstract

In vitro flowering and an efficient micropropagation protocol was developed for the annual medicinal herb Physalis minima (Solanaceae) by the in vitro culture of nodal segments of mature plant. Murashige and Skoog’s (MS)medium, 3% sucrose and0.8% agar supplemented with 4.0mg/l BAP and 0.25mg/l 2,4 - D and1.0mg/l KIN and 0.25mg/l NAA were found to develop fully functional flowers. MS medium supplemented with 2.0mg/l BAP and 0.25mg/l IAA induced an average of three shoots per explants. Full strength MS medium with 2.0mg/l IBA exhibited the best in in vitro rooting. Seventy percent of the rooted shoots survived when transferred to green house and next to the field condition.

E.Sheeba, S.Palanivel, S.Parvathi2

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