Research Article Open Access

MOLECULAR PROFILING & RAPD ANALYSIS OF COMMERCIAL HYBRID PARENTAL LINES IN TOMATO & CHILI

Abstract

In this study, tomato (Solanum lycopersicum) and Chilli (Capsicum) F1 hybrids and their parents were studied for identification and genetic purity testing using 22 and 15 random10-base oligonucleotide RAPD primers respectively. The present study analyzed suitability of RAPD markers for characterization of chilli(capsicum) hybrids and their parents. Out of 15 decamer random primers used for analysis, three primes, OPC-08, OPL-12 and OPV-14 yielded scorable results in the form of amplified products. This study also analyzed Solanum lycopersicum F1 hybrids and its parents (male and female) with 22 different decamer primers to identify the band pattern which could differentiate between the parents and hybrids. Out of these 22 RAPD primers only 5 primers, OPA-05, OPA-09, OPN- 14, OPG-04 and OPK-02 showed polymorphic bands between male, female and hybrids. Since a lot of chances are there for contamination by selfing of female parental lines and out crossing with other plants during hybrid development programs. This seriously affects the hybrid production quality. So molecular marker tools can be effectively used to find out such contaminations in DNA polymorphism of respective hybrids

Divya Pal, Madhulika Singh

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