Research Article Open Access

Prevalence of Extended Spectrum β-Lactamase and AmpC β-Lactamase among Enterobacteriaceae and Pseudomonadaceae Isolated at Tertiary Care Set up in Tripura, India

Abstract

β-lactam antibiotics are the most widely used chemotherapeutic agents. Commonest cause of resistance towards β-lactam antibiotics is the production of β-lactamases. Isolation, identification and antibiogram of the members of Enterobacteriaceae and Pseudomonaceae from the clinical samples. Detection of Extended spectrum β-Lactamase and AmpC β-Lactamase producing Enterobacteriaceae and Pseudomonadaceae by phenotypic method. Culture isolation, identification and antibiogram of the isolates were performed followed by detection of Extended spectrum β-Lactamase by Double Disc Synergy Test and Phenotypic disc confirmatory test, whereas AmpC β-Lactamase was detected by Disc Antagonism Test and Double Disc Synergy Test. Out of 200 isolates, 54% are Extended spectrum β-Lactamase producers, 48.5% are AmpC β-Lactamase producers. Predominant ESBLs producers are Klebsiella pneumonia and Escherichia coli. In case of Pseudomonadaceae, 88% of Pseudomonas aeruginosa and 100% of Pseudomonas fluorescens has shown presence of AmpC β-Lactamase and 66% of Providencia alcalifaciens produced combined Extended spectrum β-Lactamase and AmpC β-Lactamase. Out of the 97AmpC β-Lactamase producers, 45.4% and 54.6% are inducible and plasmid mediated AmpC β-Lactamase producers respectively. This study shows high rate of circulating extended spectrum β-Lactamase and AmpC β-Lactamase producers in our hospital setup. Development of antimicrobial stewardship program based on the local epidemiological data and national guidelines is the need of the hour.

Tapan Majumdar, Shibabrata Bhattacharya and Raunak Bir

To read the full article Download Full Article | Visit Full Article