Research Article Open Access

Purification of mCRP35-47 Peptide Related Antibody by Affinity Chromatography on Antigen-Ligand Columns

Abstract

To purify mCRP35-47 autoantibody from the lupus nephritis for estab-lishing the foundation of the further study on the roles of mCRP35-47 in the clinical course of lupus nephritis. The first step is obtaining the serum of patients with lupus nephritis and purifying of plasma total IgG by affinity chro-matograph. The second step is activating the sepharose with CNBr. The most important step is preparing the immobilized ligand by coupling the peptide a.a.35-47 to the gravity column and the total IgG is flow though the gravity column and can be eluted. mCRP35-47 peptide related antibody is harvest-ed and its specificity has been verified by SPR. Human mCRP35-47 peptide related antibody with high purity from the plasma can be obtained by affinity chromatography and can be applied to further functional experiments.

Qiu-yu LI, Hai-yun LI, Yi-ming WU, Feng YU, Ming-hui ZHAO

To read the full article Download Full Article