Review Article Open Access

Review of High Performance Liquid Chromatography and Its Applications

Abstract

A review of High-Performance Liquid Chromatography included an introduction, chromatographic terms, different classes, and types of HPLC techniques. Also included a brief introduction to HPLC principle and instrumentation. Columns, pumps, and detectors used in HPLC are also included in detail with their efficiency. Applications of the HPLC and overall new advantage cues are included in this review article. High Performance Liquid Chromatography (HPLC) is an important qualitative and quantitative technique, generally used for the estimation of pharmaceutical and biological samples.

The chromatography term is derived from the Greek words namely chroma (color) and graphein (to write). Chromatography is defined as a set of techniques that are used for the separation of constituents in a mixture. This technique involves 2 phases stationary phase and a mobile phase. The separation of constituents is based on the difference between partition coefficients of the two phases. Chromatography is a very popular technique and it is mostly used analytically. There are different types of chromatographic techniques namely Paper Chromatography, Thin Layer Chromatography (TLC), Gas Chromatography, Liquid Chromatography, Ion exchange Chromatography, and lastly High Performance Liquid Chromatography (HPLC). This review is mainly based on the HPLC technique its principle, types, instrumentation, and applications. The mixture is separated using the basic principle of column chromatography and then identified and quantified by spectroscopy. In the 1960s, the column chromatography LC with its low-pressure suitable glass columns was further developed to the HPLC with its high-pressure adapted metal columns.

Rushikesh Bachhav*, Piyush Bachhav, Mayur Bhamare, Ruchita Bachhav, Ganesh Sonawane, Kajal Pansare, Dhananjay Patil

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