Research Article Open Access

Simultaneous Estimation of Menthol, Camphor and Methyl Salicylate in Anti-Arthritic Spray Using Gas Chromatography

Abstract

A simple, rapid, accurate, precise and robust Gas Chromatographic (GC) method has been developed for the simultaneous estimation of Menthol, Camphor and Methyl Salicylate from an anti-arthritic spray. The separation of these three constituents was performed on DB-23 column (60 m × 250 µm × 0.25 µm). The Nitrogen was used as carrier gas at a flow rate of 2.5 mL/min. The inlet temperature and detector temperature were set as 250°C and 280°C respectively. The initial oven temperatures was 125°C for 5 min, then increase to 150°C at the ramp of 3°C/min and finally reach to 200°C at the ramp of 10°C/min. The ALS (Automatic Liquid Sampler) system was used for sample injection. The injection volume was 2 µl. The run time of chromatogram was 20.333 min. The proposed GC method provides a good resolution of Menthol, Camphor and Methyl Salicylate. The method was validated as per ICH guidelines. The linearity of Menthol, Camphor and Methyl Salicylate were found to be in the range of 10-50 µg/mL, 20-60 µg/mL and 100-300 µg/mL respectively. The recovery was calculated by using external standard method and the mean average recovery for Menthol, Camphor and Methyl Salicylate were found to be 100.55%, 99.93% and 99.95%. The tested validation parameters were found to be within acceptable limits. The method was successfully applied for quantification of these three ingredients in anti-arthritic spray formulation.

Munshi Mahammed Tanzil Mahammed Aslam, Saisivam Srinivasan, Rabadiya Vibhuti Amitkumar, Shailendra K. Mehta

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