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Suitability of Goat Tracheal Muscle Preparation for Evaluating Substances with Bronchodilator Activity

Abstract

Bronchial asthma is a disease of airways that is characterized by hyper responsiveness of the tracheobronchial tree to a multiplicity of stimuli. Testing of drug action on tracheobronchial smooth muscle should be initially done with tracheal or bronchial tissue in an organ bath by conventional methods. The present study was done to evaluate the suitability of goat tracheal muscle preparation for screening the drugs or substances with bronchodilator activity. Goat trachea was collected from the slaughter house from a freshly slaughtered goat. A series of dose responses with different doses of histamine were obtained till the ceiling dose was obtained. Varying doses of theophylline anhydrous which was prepared by dissolving in warm distilled water were added to the bath and allowed to act. It was observed that theophylline anhydrous (5X10-3) solution used antagonized histamine induced contraction responses (p<0.001). The goat tracheal muscle preparations are easier to handle, prepare and also seems to be more sensitive than guinea pig tracheal chain with consistent and dependable responses. This preparation can be used for screening variety of plant extracts or products to evaluate the bronchodilator activity. The goat tracheal preparation is very easily available and sacrificing laboratory animals in large numbers can be avoided for evaluation of substances with bronchodilator property.

Niveditha G

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