Research Article Open Access

The Comparison Between the Cytotoxic Effect of Deinoxanthin and Mitomycin-C on MCF7 Cell Lines and the Changes on Bax and BCL2 Genes After Exposure

Abstract

Background: Deinoxanthin, is a carotenoid extract isolated from Deinococcus radiodurans. Mitomycin-c, as a cancer chemotherapeutic agent, has been used for the treatment of wide ranges of malignancies including but not limited to uterus, lung, chest, stomach and bladder cancer for over 30 years.

Purpose: The aim of this study was to investigate the apoptotic effect of deinoxanthin on MCF7 cell lines compared to mitomycin-c.

Method: Apoptotic effects were evaluated in MCF₇ cells and were measured through cell viability and morphological changes. Cellular viability was measured using MTT assay and the expression of apoptotic and anti-apoptotic genes was assayed by real-time PCR.

Results: The half-maximal inhibitory concentration (IC₅₀) values for deinoxanthin against the MCF7 cell line after 24, 48 and 72 hours after treatments were 18.18, 22.18, 21.18 μg/ml and the IC₅₀ values for mitomycin-c were 2.26, 0.96, 1.96 μg/ml. Moreover, BCL2 and Bax expressions in MCF₇ cell line increased by treatment with mitomycin-c, deinoxanthin and both of these cytotoxic agents.

Conclusion: The present findings demonstrate the novel functional property of deinoxanthin isolated from radioresistant bacteria as a potent inducer of apoptosis in MCF₇ breast cancer cell line. These data suggest that deinoxanthin could be potentially useful as a chemopreventive agent of the ketamine.

Minoo Iranshahi* and Tahere Naji

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