Research & Reviews: Journal of Microbiology and Biotechnology
MenuE- ISSN: 2320 - 3528
P- ISSN: 2347 - 2286
E- ISSN: 2320 - 3528
P- ISSN: 2347 - 2286
Selda Goharkhany*, Ali Maleki, Sahra Goharkhani, Lida Lotfollahi, Nima Shaykh Baygloo, Asma Najarzadeh, Yousef Sabet and Hamed Charkhian
University of Tabriz, Tabriz, Iran Urmia University of Medical Sciences, Iran Amirkabir University of Technology, Iran University of Maragheh, Iran University of Isfahan, Iran Islamic Azad University, Iran
Posters & Accepted Abstracts: RRJMB
The spread of antibiotic resistance and failure to control hospital infections are two dangerous challenges for treatment and care systems. Infectious microorganisms especially Pseudomonas aerosinosa have acquired various ways to escape from the killing property of antibiotics either intrinsically or acquired. Scientists have proposed various solutions to solve this issue, but the simplest of them is to remove infectious agents and cut the chain of transmission. One of the potential weapons to fight infections is to take advantage of the high antibacterial potential of herbal compounds such as henna. In this study, for the first time, the synergistic potential of the ethanolic extract of Lawsonia inermis Linn (henna) and common antibiotics against Pseudomonas aeruginosa with multidrug resistance was studied. Pseudomonas aeruginosa isolates were collected from infected patients hospitalized in Urmia/Iran hospitals. After the biochemical identification of the isolates, their antibiotic sensitivity pattern was investigated. Fresh henna leaves were obtained from Rudbar/ Kerman/Iran and dried in a dark environment. After grinding the henna leaves, the extracts of the samples were extracted using a Soxhlet extractor and hydroalcoholic solvent (water + methanol). 30 microliters of 10% henna leaf extract was added on the antibiotic discs and the plates were incubated for 24 hours at 37�?°C. Growth of 2 to 6 mm in the diameter of the inhibition zone of antibiotics was observed. The promising results of this study can help the therapeutic systems in hospital infection control and control of resistant microbial agents.