Research & Reviews: Journal of Microbiology and Biotechnology
MenuE- ISSN: 2320 - 3528
P- ISSN: 2347 - 2286
E- ISSN: 2320 - 3528
P- ISSN: 2347 - 2286
Promod K Mehta*, Bhawna Dahiya, Tulika Prasad, Anam Rais, Aishwarya Soni and Suman Sharma
Maharshi Dayanand University, India Jawaharlal Nehru University, India Postgraduate Institute of Medical Sciences, India
ScientificTracks Abstracts: RRJMB
Tuberculosis (TB) is the leading cause of ill health globally, which is now considered as the second major killer from an infectious agent after COVID-19 pandemic. Extra pulmonary tuberculosis (EPTB) accounts for ~4.5-47.9% of total TB burden that comprised of TB lymphadenitis, TB pleurisy, urogenital TB, abdominal TB, etc. Although diagnosis of pulmonary TB is significantly established, EPTB diagnosis is an onerous task owing to diverse anatomical locations, inconsistent clinical presentations and sparse bacillary load in clinical specimens. We recently designed a nano-based immunoassay i.e. magnetic bead-coupled gold nanoparticlebased SYBR Green real-time immuno-PCR (MB-AuNP-RT-IPCR, liquid system) for the quantitative detection of MPT- 64+CFP-10 proteins in clinically suspected EPTB patients and those results were compared with gel-based MB-AuNP-I-PCR and Magneto-ELISA. A wide range (270 fg/mL to 9.9 ng/mL) of MPT-64+CFP-10 was quantified by MB-AuNP-RT-I-PCR in EPTB patients, while Magneto-ELISA showed a narrow range (1.8-10 ng/mL) of the same. Subsequently, a high sensitivity (88.2%) and specificity (100%) was acquired by SYBR Green MB-AuNP-RT-I-PCR in clinically suspected EPTB patients (n=51) and non-TB control subjects (n=49). Of note, both MB-AuNP-I-PCR and Magneto-ELISA exhibited significantly lower (p<0.05-0.01) sensitivities than MB-AuNP-RT-I-PCR. Our MB-AuNP-RT-I-PCR exhibited a good diagnostic yield, which may translate into a prospective diagnostic kit..
Promod K Mehta is currently working as a Professor at Amity University of Rajasthan, Jaipur, India since March, 2023. He earned his PhD (Microbiology) degree from Panjab University, Chandigarh, India in 1988 and joined as a Lecturer (Microbiology) in Delhi University College and continued till 2009 as an Associate Professor. During this tenure, he did rigorous postdoctoral research training at the Centers for Disease Control and Prevention, Atlanta, USA (1994-96) and the University of Nebraska- Lincoln, USA (1999-01). He worked as a Professor (2009-2019), Centre for Biotechnology (CBT), Maharshi Dayanand University (MDU), Rohtak, India and later as an Emeritus Scientist (2019-2023). He also worked as a Director, CBT, MDU, Rohtak for three years (2015-18). He worked extensively on several aspects of TB pathogenesis, and is presently focusing his research on ‘anti-TB drug designing’ and ‘TB diagnostics’ use novel molecular and immunological tools. He has published 57 research papers in reputed international journals.