Lactobacillus acidophilus producing bacteriocin/bacteriocin-like compounds supernatants as a highly potent inhibitor of the pathogenic bacteria growth have significant toxicity in HepG-2 cancer cell lines

Joint Webinar: 26^th World Congress on Advanced Biotechnology & 3^rd World Congress on Food Safety and Nutrition Science

April 27, 2023 | Webinar

Mehran Amir Javid*, Hamed Charkhian, Arash Adamnejad ghafour, Selda Goharkhany, Lida Lotfollahi, Seref Bugra Tuncer, Parisa Hosseini, Ali Maleki, Nesa Yousefi and Mohammad Hossein Sadeghi Zal

Islamic Azad University, Iran
Istanbul University, Turkey
University of Tabriz, Iran
Urmia University of Medical Sciences, Iran

ScientificTracks Abstracts: RRJMB

Abstract

Background: Cancer, as the second cause of human death and one of the health important problems, brings enormous social, economic, and spiritual costs to human society. Although chemotherapy is one of the most effective methods for cancer treatment, but side effects and the development of treatment resistance in tumor cells are among the most critical limitations of this method. The bacteriocins, including peptides belonging to the Lactobacillus family, are one of the newest anticancer sources that have attracted the attention of researchers. Methods: In a cross-sectional study, 150 Staphylococcus epidermidis strains were isolated from different Urmia/ Iranian clinical sources during a three month period. The isolates were investigated by Spot test and Well diffusion method for the ability to produce antibacterial compounds. The bacteriocins/BLISs production was induced by mitomycin C. HepG-2 cells were incubated for 24 hours with different concentrations of bacterial supernatant containing the strongest antibacterial compounds. The degree of toxicity was measured through the MTT dye test (cell viability) and trypan blue dye exclusion assay (cell mortality). Results: 23 isolated from the S. epidermidis had potent activity against Bacillus cereus, Listeria monocytogenes, Klebsiella spp., Staphylococcus aureus, P. aeruginosa, Proteus spp., and Vibrio parahaemolyticus. The antibacterial peptides were heat-stable up to 100�?°C and active within a pH range of 3-9. The antibacterial peptides production started in the mid-exponential phase of growth and the maximum level occurred in the late-stationary phase after 30 hours. The pure supernatant of Lactobacillus acidophilus has the ability to inhibit the growth of HepG-2 cell lines, and the death rate of cancer cells increases with increasing concentration. Conclusions: Due to the potential of the supernatant containing the bacteriocins/BLISs produced by Lactobacillus acidophilus on HepG-2 cancer cell lines, we can be used them as an auxiliary and complementary medicine in the treatment process of cancer patients.

Biography

Mehran Amir Javid graduate of Microbial Biotechnology from Salamic Azad University Urmia/Iran. He is very interested in microorganisms and their metabolites, because these microscopic organisms have thousands of interesting peptides. For this purpose, he did his thesis on the topic of anticancer metabolites of microorganisms, and now he is completing his studies for different research groups.

• PDF
• HTML