Research & Reviews: Journal of Material Sciences
MenuISSN:2321-6212
ISSN:2321-6212
Alberto Jorge-Mora, Jesus Pino-Minguez, Rodolfo Gomez-Vaamonde and Daniel Nieto
Universidad de Santiago de Compostela
Santiago University Clinical Hospital, Spain
Universidad de Santiago de Compostela, Campus Vida, Spain
ScientificTracks Abstracts: Res. Rev. J Mat. Sci.
Orthopaedic surgery has grown from the hand of new materials that made possible to perform procedures as total hip replacement with feasibility. These procedures are common in most orthopaedic departments, and more than 70 000 hip or knee replacements are performed in Spain every year. The survival of these implants is critical to prevent loosening and the need for revision arthroplasty. The ideal surface to interact with bone has not been created. We created laser induced periodic surface structures (LIPSS) in the surface of titanium and tantalium to study the behaviour of stem cells compared to polished surfaces. We created 12 discs of each material and polished them. Later we created LIPSS in 6 discs of each material. We cultured them in human stem cells in a concentration of 25000 cell per cm2 for 20 days. We determined MTT, TNF-Alfa, alkaline phosphatase, IL-6, osteopontin and osteocalcin every 5 days until the day 20. We confirmed outcomes behave as a normal distribution after applying the Kolmogorov Smirnov test. We compared materials and surfaces with the T-student test. We accepted a difference of 0.05 as significant. LIPSS created increase statistically cell metabolism (best values in MTT assay) and decrease inflammatory response to the material (IL-6 and TNF-alfa values). Collagen is produced in more quantity and cells differentiate to osteoblast easily. These differences are seen from the beginning until the endpoint (day 20). When LIPSS improved osteogenic properties of titanium and tantalium compared to smooth surfaces.
Alberto Jorge is an orthopaedic Surgeon with special interest in biomaterials. He is focusing every effort in the study of the behaviour of surface modifications in the field of orthopaedic surgery. Previously he performed different modifications in PMMA to create composites with hydroxyapatite to prevent fibroblastic response (Pino et al 2015). Nowadays he is dedicated to laser modification of metals and cermets to increase anisotropic osteointegration of implants.