Research & Reviews: Journal of Microbiology and Biotechnology
MenuE- ISSN: 2320 - 3528
P- ISSN: 2347 - 2286
E- ISSN: 2320 - 3528
P- ISSN: 2347 - 2286
Tulcan Camelia
King Michael I of Romania, Romania
Posters & Accepted Abstracts: JMB
Introduction and aim: Polyphenols are secondary metabolites of plants, being a large and diverse group of substances present abundantly in all medicinal plants. They have antioxidant, anti-inflammatory and biological effects that are manifested in the prevention of pathological conditions. Polyphenols exhibit strong antioxidant activity due to their high reactivity towards the reactive oxygen species. Over time it has been demonstrated that their reactivity is improved under alkaline conditions. The extraction procedures of polyphenols involve several steps of which, probably the most important is the selection of a suitable solvent to ensure an optimal extraction percentage of the bio-constituents. A number of factors in the processing procedure affect the total phenolic content. Liquid-liquid and solid-liquid extraction methods are still the most commonly used extraction procedures. The alkaline electrolyzed water is recognized for its remarkable solvent properties, providing a very good extraction yield in particular on vegetal matrices. Due to its capacity to exhibit the alkaline power of hydrogen, the alkaline electrolyzed water also possesses the ability to scavenge reactive oxygen species. The purpose of this study was to analyze how the use of alkaline electrolyzed water at different pH (8,5; 9; 9,5 and 11,5) influences the quality and quantity of extracted polyphenols from Hysoppus officinalis, Allium ursinum, Chelidonium Majus and Thymus Serpyllum extracted in distinctive working conditions (stirring and ultrasonication). The results indicated a significant extraction capacity of the alkaline water regarding the compounds of interest, especially at pH 9, by ultrasonication.
Camelia Tulcan is the Associate Professor at Biochemistry Department, Faculty of Veterinary Medicine, Timisoara and Coordinator of Antioxidant Research Lab-Horia Cernescu Research Unit. She has expertise in oxidative stress evaluation in different physiological or pathological condition and is involved in management team of research and infrastructure projects and in implementation of quality management systems.