Transient expression of monoclonal antibody and bispecific fragments and fragment/antigen complexes for pharmaceutical discovery research

Joint Event on 15^th International Conference and Exhibition on Materials Science and Engineering & 3^rd International Conference on Applied Crystallography

November 07-08, 2018 | Atlanta, USA

Ramesh Iyer

AbbVie Inc., USA

ScientificTracks Abstracts: Res. Rev. J Mat. Sci

DOI: 10.4172/2321-6212-C8-035

Abstract

Fragments, such as F(ab) and F(ab�)2 and fragment complexes are widely required for various research and development activities in biopharmaceutical discovery. We describe the production of fragments (F(ab) and F(ab�)2) from a variety of parent molecules (monoclonal antibody and DVD-Ig) and frameworks (mIgG1, mIgG2a, huIgG1, huIgG4) by transient expression in HEK293 cells.The method eliminates the need to use enzyme digestion of parent molecules. We have also developed a route for the production of fragment/antigen complexes by direct co-expression in HEK293 for crystallization purpose that allows for higher throughput screening of F(ab) and/or antigen constructs. The resulting products were characterized by biophysical techniques and crystallization experiments demonstrated that the fragments and fragment/antigen complexes produced diffraction quality crystals suitable for X-ray crystallographic analysis.

Biography

Ramesh Iyer has completed his PhD from the University of Kentucky (Lexington, KY) and Postdoctoral studies from the University of Georgia (Athens, GA). He is a Sr Scientist in the Global Biologics group at AbbVie Inc. His group supports the research and development of biologics for various therapeutic areas within AbbVie Discovery.

E-mail: ramesh.iyer@abbvie.com

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