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conferenceseries
.com
Volume 5, Issue 6 (Suppl)
J Mat. Sci.
ISSN: 2321-6212
Advanced Materials 2017
October 26-28, 2017
OCTOBER 26-28, 2017 OSAKA, JAPAN
13
TH
INTERNATIONAL CONFERENCE ON
Advanced Materials and Nanotechnology
Preparation of the SAHA-Pluronic F127 nanoparticles and anticancer activity
in vitro
Xiong Wang
1
, Xiao Zhang
1
, Manli Li
1
, Ping Zong
1
, Tingting Kong
1,2
, Yanzhen Hu
1
, Liyuan Liang
1
and Chao Li
1
1
Xi’an Jiaotong University, China
2
Suzhou GuanJie Nano Antibacterial Coating Technology Co. Ltd., China
Purpose:
Vorinostat (SAHA) is the most representative histone deacetylase inhibitor and a widely used anticancer drug,
SAHA is applied in the treatment of hematological malignancies and most solid tumors. SAHA is challenging due to poor
water solubility, low bioavailability and rapid elimination of drugs
in vivo
. In this study, we will prepare SAHA-Pluronic F127
Nanoparticles and investigated whether this could improve drug solubility, the effect of sustained release and inhibitory effect
on cancer cells.
Methods:
SAHA-Pluronic F127 nanoparticles were prepared by thin-film method. The drug loading, entrapment efficiency
and
in vitro
drug release test were determined by High Performance Liquid Chromatography (HPLC). The proliferation on
HeLa cell was examined by MTT. The mRNA expression of p53 and p21 were determined by real-time PCR.
Results:
The particle size of the nanoparticles was 23.86±0.30 nm, the encapsulation efficiency was 94.36±0.76% and the drug
loading was 1.31±0.062. The results showed that SAHA NPS can achieve about 17% sustained release effect and the inhibitory
effect on cell growth of SAHA NPS was better than SAHA. Both the SAHA and SAHA NPS up-regulated p21 and down-
regulated p53 in mRNA level after 24 h and 48 h treatment and SAHA nanoparticles had better effects compared to SAHA at
48 h.
Conclusion:
This study confirmed encapsulation of SAHA into Pluronic F127 nanoparticles can improve drug aqueous
solubility and sustained release in vitro and the inhibition of cell growth of SAHA NPS was more potent than SAHA.
Biography
Xiong Wang has completed his Bachelor's degree in Pharmacy from Wenzhou Medical University. Presently, he is pursuing his Master’s degree from the Xi'an
Jiaotong University, China.
w18862117607@yahoo.comXiong Wang et al., J Mat. Sci. 2017, 5:6
DOI: 10.4172/2321-6212-C1-009