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Volume 5, Issue 5

Res. Rev. J Mat. Sci. 2017

ISSN: 2321-6212

Advanced Materials 2017

September 07-08, 2017

September 07-08, 2017 | Edinburgh, Scotland

Advanced materials & Processing

International Conference on

Dual-faced nano-mushrooms for tri-functional single cell diagnosis and drug delivery

1Hsin-Yi Hsieh,

Tsu-Wei Huang,

Chau-Hwang Lee

and

1,2,3

Fan-Gang Tseng

National Tsing Hua Univerity, Taiwan

Applied Science Research Center and Academia Sinica, Taiwan

ere we introduce a monodispersed mushroom-like fluorescent nanoparticle with dual-faces and tri-functions for SERS-active

Raman sensing, fluorescence detecting, cancermarker targeting, and drug carrying and delivering inside a cell. Aone-step oxygen

plasma process was employed to tailor commercial-available fluorescent PS beads into corrugated hemispheres and simultaneously

modify the entire surface with carboxylic groups, and then a gold film was coated on the corrugated hemisphere for SEARs. Sulfo-

NHS-SS-biotin disulfide linker and anti-CD44 monoclonal antibody could be modified simultaneously onto the top gold surfaces

and bottom carboxyl groups through Au-S and peptide bonds, respectively. In exploiting the dual-module surface, highly selective

modifications were performed to the Au-S bond using thiols and/or the peptide bond using a dehydration reaction between –COOH

and –NH

. For applications in cancer, the DFPSBs were modified by attaching anti-CD44 antibody (on the carboxylated polystyrene)

and a sulfo-NHS-SS-biotin disulfide linker (onto the amine or gold surface). The anti-CD44-modified DFPSBs can be utilized to

target cancer cells (such as HeLa and MCF-7) with CD44 over-expressed. For drug delivery, a relatively weak covalent bond in the

disulfide linker has the advantage of being capable of cleavage via reduction. The disulfide cleavage, dividing one R-SS-R into two

R-SH molecules, occurs with cell cytoplasm environment. In the intracellular space, the cell regulatory mechanism can retain the

redox equilibrium; consequently, the disulfide linker-modified DFPSBs act as vehicles releasing their load inside the cell membrane.

Therefore, surface-modified DFPSBs can integrate three functions on the nanoparticles for biomedical applications.

Res. Rev. J Mat. Sci. 2017, 5:5

DOI: 10.4172/2321-6212-C1-006