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Volume 5, Issue 5
Res. Rev. J Mat. Sci. 2017
ISSN: 2321-6212
Advanced Materials 2017
September 07-08, 2017
September 07-08, 2017 | Edinburgh, Scotland
Advanced materials & Processing
11
th
International Conference on
Bioceramics and their applicability in dentistry
Csaba Hegedus, Melinda Szaloki, Kinga Bagyi, Viktoria
Hegedus and Jozsef Bako
University of Debrecen, Hungary
S
tatement of the Problem: Different type of ceramic scaffold materials are widely used in dentistry, oral surgery and implantology.
The most often used are hydroxyapatite (HA) and -tricalcium phosphate (ßTCP) bioactive modified scaffolds among the ceramic
scaffolds. The appropriate mechanical properties, capable of supporting cell attachment and proliferation are critical parameters at the
designing of a scaffold. Moreover, porosity, themechanical integrity and effect of surfacemorphology on cell adhesion and proliferation
are important parameters that must be examined in constructing the scaffold. The purpose of this study is to characterize and
investigate the possible dental application of HA and ßTCP containing mesoporous silica based aerogel. Methodology & Theoretical
Orientation: Modified aerogels with different HA/βTCP ratio were fabricated by sol-gel technology and supercritical drying in carbon
dioxide at 80
o
C. In our in vitvo study the cytotoxicity of modified aerogel was analysed based on Alamar Blue assay (Invitrogen, USA)
by using malignant osteosarcoma cell line (SAOS-2, ATCC® HFB- 85™, Rockville, MD, USA). The gene expression changes were
measured with BMP-2, Runx2, TaqMan® assays (ABI, USA), normalized to the GAPDH level. In our in vivo experiments modified
aerogels discs were implanted into the prepared rat calvaria defects to investigate the osteointegration around the inserted aerogels.
The thickness of prepared histological bone section containing modified aerogel were under 10 micrometres and stained based on
the haematoxylin-eosin (H&E) staining protocol. Findings: These measurements showed that modified aerogels are biocompatible
and non-toxic for SAOS-2 cell type. The gene expression analysis showed that ßTCP and/or HA containing composites can trigger
differentiation of SAOS-2 cells to osteoblasts, however, ßTCP containing aerogel looks more effective than the HA containing aerogel.
In vivo experiments showed early signs of osteointegration after 1 month intervention. Conclusion & Significance: These materials
showed promising properties to be useful scaffolds for bone regeneration.
hegedus.csaba.prof@dental.unideb.huRes. Rev. J Mat. Sci. 2017, 5:5
DOI: 10.4172/2321-6212-C1-006