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Volume 5, Issue 6 (Suppl) J Mat. Sci.
ISSN: 2321-6212 Advanced Materials 2017
October 26-28, 2017
OCTOBER 26-28, 2017 OSAKA, JAPAN
13TH INTERNATIONAL CONFERENCE ON
Advanced Materials and Nanotechnology
Rapid, simple detection of oseltamivir-resistant influenza-A virus (H275Y mutation) using gold
nanoparticles-based colorimetric probe capable of specific recognition to the H275Y NA mutation
Eun-Kyung Lim
Korea Research Institute of Bioscience and Biotechnology, Republic of Korea
Recently, an oseltamivir-resistant pandemic H1N1 virus has emerged because of the increased use of oseltamivir. This
oseltamivir-resistant virus has spread around the world and is being highly contagious. Therefore, for immediate virus
control and prevention, accurate detection techniques that can rapidly identify oseltamivir-resistant virus are required. The
currently used assays, which are dependent on genomic-based assays because of drug-resistant viruses caused by a genomic
mutation in viral Neuraminidase (NA) (H275Y mutation), are time consuming and require trained personnel for sample
preparation and analysis. We have developed a novel nanoprobe consisting of Gold Nanoparticles (GNPs) modified with an
oseltamivir derivative (oseltamivir hexylthiol; OHT) as a targeting moiety (OHT-GNPs) enabling a simple colorimetric assay
for oseltamivir-resistant virus (H275Y mutation) detection. This assay is based on the colorimetric change of GNPs in the
presence of oseltamivir-resistant virus due to the specific interaction with OHT of nanoprobe (OHT-GNPs) and the mutated
viral NA site. OHT exhibited 250-fold-higher binding affinity to the NA site of the mutant virus (H275Y mutation) than to
the Wild-Type Virus (WT) according to NA activity and modeling analysis. Our results showed a color change of OHT-GNPs
from deep red to purple because of OHT-GNPs aggregation by interacting with OHT structure of OHT-GNPs and NA on the
surface of H275Y mutant virus. Especially, these colors were gradually changed to purple as the mutant virus concentration
increased, which allowed determining the presence of the virus with the naked eye. Using OHT-GNPs, it was possible to
sensitively detect oseltamivir-resistant virus (H275Y mutation) at low virus concentrations by changing its absorbance; the
Limit of Detection (LOD) of this assay was 10 Plaque-Forming Units (PFU). Furthermore, OHT-GNPs-based dipstick assay
could detect H275Y mutation as well as obtain their qualitative analysis. This assay may provide accurate information whether
this virus is oseltamivir resistant that could support choosing appropriate treatments using Point-of-Care (POC) diagnostics.
Biography
Eun-Kyung Lim has completed her PhD degree in chemical engineering from Yonsei University, Republic of Korea. She has joined Bio-nano technology Research
Center in KRIBB. Her research efforts are on development of smart theranostics nanosystems for cancer metabolism regulation and sensing nanoprobe for
bacteria/virus.
Eun-Kyung Lim, J Mat. Sci. 2017, 5:6
DOI: 10.4172/2321-6212-C1-009