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Volume 5, Issue 5 Res. Rev. J Mat. Sci. 2017
ISSN: 2321-6212 Advanced Materials 2017
September 07-08, 2017
September 07-08, 2017 | Edinburgh, Scotland
Advanced materials & Processing
11th International Conference on
LIPSS stimulate stem mesenchymal cells differentiation to osteoblasts in titanium and tantalium
Alberto Jorge-Mora1, Jesús Pino-Mínguez2, Rodolfo Gomez-Vaamonde3 and Daniel Nieto4
1, 2Universidad de Santiago de Compostela
3Santiago University Clinical Hospital, Spain
4Universidad de Santiago de Compostela, Campus Vida, Spain
Orthopaedic surgery has grown from the hand of newmaterials that made possible to perform procedures as total hip replacement
with feasibility. These procedures are common in most orthopaedic departments, and more than 70 000 hip or knee replacements
are performed in Spain every year. The survival of these implants is critical to prevent loosening and the need for revision arthroplasty.
The ideal surface to interact with bone has not been created. We created laser induced periodic surface structures (LIPSS) in the surface
of titanium and tantalium to study the behaviour of stem cells compared to polished surfaces. We created 12 discs of each material
and polished them. Later we created LIPSS in 6 discs of each material. We cultured them in human stem cells in a concentration of
25000 cell per cm2 for 20 days. We determined MTT, TNF-Alfa, alkaline phosphatase, IL-6, osteopontin and osteocalcin every 5 days
until the day 20. We confirmed outcomes behave as a normal distribution after applying the Kolmogorov Smirnov test. We compared
materials and surfaces with the T-student test. We accepted a difference of 0.05 as significant. LIPSS created increase statistically cell
metabolism (best values in MTT assay) and decrease inflammatory response to the material (IL-6 and TNF-alfa values). Collagen is
produced in more quantity and cells differentiate to osteoblast easily. These differences are seen from the beginning until the endpoint
(day 20). When LIPSS improved osteogenic properties of titanium and tantalium compared to smooth surfaces.
Biography
Alberto Jorge is an orthopaedic Surgeon with special interest in biomaterials. He is focusing every effort in the study of the behaviour of surface modifications in
the field of orthopaedic surgery. Previously he performed different modifications in PMMA to create composites with hydroxyapatite to prevent fibroblastic response
(Pino et al 2015). Nowadays he is dedicated to laser modification of metals and cermets to increase anisotropic osteointegration of implants.
Alberto Jorge-Mora et al., Res. Rev. J Mat. Sci. 2017, 5:5
DOI: 10.4172/2321-6212-C1-005