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Pathology 2018
Research & Reviews: Journal of Medical and Health Sciences
ISSN: 2319-9865
Page 65
October 08-09, 2018
Edinburgh, Scotland
17
th
International Conference on
Pathology & Cancer
Epidemiology
M
echanisms of cement dust induced toxicity in lung cells have
been scarcely studied. In the present study, we investigated
endocytosis of cement dust and clinker particles, apoptosis,
mitochondrial membrane potential (∆Ψ
m
) alteration, NADP+,
NADPH and NADP/NADPH ratio,
in situ
DNA fragmentation
and oxidative stress in human alveolar type II epithelial cells
(A549) exposed to cement dust particles. The endocytosis of
particles was evaluated using transmission electron microscope
(TEM), 3-(4, 5-dimethylthiazol-2-yl)-2, 5 diphenyl tetrazolium
bromide (MTT) assay, LDH leakage, NADP/NADPH by enzyme
linked immunosorbent assay (ELISA) and ∆Ψ
m
using JC-1 dye.
Additionally, apoptosis, intracellular reactive oxygen species
(ROS) production and reduction of intracellular reduced
glutathione (GSH) were quantified using flow cytometry method,
while in situ DNA strand break was examined using TUNEL
assay. Alveolar epithelial cells evaluation shows endocytosis of
cement dust or clinker particles into the cytoplasmic vacuoles.
Though, cells exposed to clinker were found to internalized
clinker predominantly at the membrane bound vacuoles. Also,
both cement dust samples and clinker induced reduction of ∆Ψ
m
and NADP/NADPH ratio with subsequent increased induction
of apoptosis and ROS generation. All dust samples induced
profound reductionNADP/NADPH ratiowhich suggests reduction
in intracellular reducing power. Collectively, data from this study
show that endocytosis of cement dust and subsequent reduction
of ∆Ψ
m
and NADP/NADPH ratio, increased apoptosis, intracellular
ROS production and reduction of GSH in type II epithelial cells
may be key mechanisms of cement dust induced lung cells
damage and may contribute significantly to cement dust induced
lung fibrosis.
aookorod@utmb.eduEndocytosis of cement dust exposure as a cause of altered
mitochondrial membrane potential, apoptosis, NADP/NADPH and
oxidative stress in human type II epithelial cells
Ogunbileje J O
1
, Bazuaye Ekwuyasi A E
2
and
Okorodudu A O
1
University of Texas Medical Branch, Texas
RRJMHS 2018
Volume: 7