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Pathology 2018
Research & Reviews: Journal of Medical and Health Sciences
ISSN: 2319-9865
Page 61
October 08-09, 2018
Edinburgh, Scotland
17
th
International Conference on
Pathology & Cancer
Epidemiology
Background:
Bladder cancer even in early stage develop
recurrence. Poor sensitivity of cytology and invasiveness of
urethrocystoscopy have generated interest in non-invasive tools
to monitor for recurrence. Caspase-3 and survivin have central
role in regulation of apoptosis. Survivin can aid early diagnosis,
determine prognosis in multiple cancer types and predict
response to anti-cancer therapies. Its combination with other
biomarkers as caspase-3 enhance prognostication and prediction
of treatment response in UBC.
Methods:
Immunohistochemical expression of survivin and
caspase-3 were assessed in 44 Egyptian consecutive patients
with UBC and 7 cystoscopic biopsies of cystitis as control reactive
benign urothelium. Relationships between their expression,
clinicopathological characteristics, diagnostic and prognostic
performance were statistically analyzed.
Findings:
No survivin immunoreactivity was identified in non-
neoplastic bladder tissue. Expression of survivin and caspase-3
was altered in 42(95.5%) and 10(22.7%) cases, respectively. There
wasstatistically significantmoderatepositivecorrelationbetween
survivin and caspase-3 expression among whole studied cases
(
p
=0.006). Expression of either survivin or caspase-3 protein
individually significantly differ (
p
=0.000) in cancer status from
control cases. Survivin was an independent predictor of UBC in
multivariable analyses. Diagnostic accuracy of survivin alone was
significantly better than caspase-3 alone (sensitivity 81.82% vs.
68.18%,
p
=0.027). Addition of survivin immunoreactivity to model
including caspase-3 expression improved diagnostic accuracy
with a sensitivity of 93.18%. Addition of gender to the previous
model improved more diagnostic accuracy with sensitivity of
100%.
Interpretation:
Survivin alone is very promising marker and
reliable indicator in UBC. Survivin and caspase-3 antigens have
a cooperative effect on bladder cancer, their simultaneous
evaluation augments diagnostic sensitivity.
Biography
Vivian G D Rouston obtained her MBBCh in 2004 and Masters in Pathology
in 2015 from Faculty of Medicine Alexandria University. She was trained for
Histopathology and Cytopathology at Histopathology Division of the De-
partment of Pathology, St James’s University Hospital, the Leeds Teaching
Hospitals, NHS Trust, United Kingdom. She is working as a Histopathology
Specialist in a general hospital in Egypt.
v_dabous@hotmail.comVivian G D Rouston, Amal A A Shaaban, Dina M Abd Allah
and
Ahmed F Kotb
Alexandria Faculty of Medicine - University of Alexandria, Egypt
Vivian G D Rouston et al., RRJMHS 2018
Volume: 7
Survivin and caspase-3 as a diagnostic and predictive
biomarkers of recurrence for urinary bladder carcinoma
after TURBT