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Volume 5, Issue 6 (Suppl) J Mat. Sci.
ISSN: 2321-6212 Advanced Materials 2017
October 26-28, 2017
OCTOBER 26-28, 2017 OSAKA, JAPAN
13TH INTERNATIONAL CONFERENCE ON
Advanced Materials and Nanotechnology
One-dimensional poly(L-lysine)-block-poly(L-threonine) assemblies as anticancer agents
Chao-Liang Wu
National Cheng Kung University, Taiwan
We synthesized cationic, one-dimensional fibril assemblies formed from coil-sheet poly(L-lysine)-block-poly(L-
threonine) (PLL-b-PLT) block co-polypeptides as anticancer agents. The 1D fibril assemblies can efficiently interact
with negatively charged cellular and mitochondrial membranes via electrostatic interactions, leading to cell necrosis through
membrane lysis and apoptosis via the lytic effect of mitochondria. This effect is similar to that of one-dimensional drug carriers
that exhibit enhanced cell penetration. Compared to free PLL chains, PLL-b-PLT fibril assemblies exhibited more selective
cytotoxicity against cancer cells, lower hemolytic activity, higher membranolytic activity and a different apoptotic pathway,
which may be due to differences in the peptide-membrane interactions. The fibril assemblies significantly inhibited tumor
growth, improved survival and suppressed tumor metastasis to the lung in C57BL/6 mice bearing syngeneic LL2 lung tumors.
An additive antitumor activity was also observed when the tumor bearing mice were treated with PLL-b-PLT in combination
with the common chemotherapeutic drug cisplatin. Collectively, these results support the feasibility of using one-dimensional
fibril assemblies as potential anticancer therapeutics.
Biography
Chao-Liang Wu is a Distinguished Professor of the Department of Biochemistry and Molecular Biology and Institute of Clinical Medicine, College of Medicine,
National Cheng Kung University, Taiwan. He has received his PhD in Molecular Biology from University of Edinburgh. He has his expertise in the areas of
translational medicine, animal models, gene therapy and nanotechnology. He has published more than 100 papers in peer-reviewed international journals.
Chao-Liang Wu, J Mat. Sci. 2017, 5:6
DOI: 10.4172/2321-6212-C1-009