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Euro Pharma Chemistry & Future Pharma 2019
Volume 08
Research & Reviews: Journal ofPharmaceutical Analysis | ISSN : 2320-0812
June 27-28, 2019 | Amsterdam, Netherlands
12th World congress on
Joint Event
4
th
Pharmaceutical Chemistry Conference Future Pharma
Phytochemical and antioxidant assessments of three fractions from methanol extract of
Spathodea campanulata Beauv. leaves
Ojah Emmanuel Onah
University of Ibadan, Nigeria
M
ethanol extract of Spathodea campanulata leaves was obtained by cold extraction, and partitioned
into hexane, ethyl acetate and methanol fractions. Phytochemical screenings of the fractions were
carried out using standard procedures to identify the class of constituents present in each of them. Ethyl
acetate fraction was subjected to column chromatographic separations by gradient elution, and isolates were
TMS (Trimethylsilyl) derivatized and characterized by GC-MS (gas chromatography-mass spectrometry).
Antioxidant content was also evaluated on the three fractions using 2, 2-diphenyl-picrylhydrazyl (DPPH)
free radical scavenging method. Percentage of inhibition and IC50 values were obtained for each fraction.
Phytochemical screenings revealed presence of alkaloids, tannins, saponin, resins, phenol, cardiac glycosides,
steroids, flavonoids, anthraquinones and terpenoids in the three fractions in varying concentrations. Alkaloids,
resins, phenol and cardiac glycosides were found to be intense in the three fractions, while phlobatannin was
found to be absent in all the three fractions. Three compounds isolated from the ethyl acetate fraction were
characterized based on MS and IR spectral interpretations as palmitic acid, ethylamine and caffeic acid.
Percentage of inhibition of the three fractions indicates that they have substantial antioxidant activity with
the standards at high concentration of 250-1000 μg/mL. The hexane fraction has the highest antioxidant
activity with an IC50 of 178.46 μg/mL when compared to other fractions; this paper reports phytochemical
constituents and high antioxidant activity (at concentrations of 250 μg/ mL and above) of the African tulip
tree (Spathodea campanulata) when compared to the standards. This has not been earlier reported in literature,
our result supports its wide ethno-medicinal applications.
Pharmaceutical Analysis 2019, Volume 08